Original articles

Research center for Molecular Identification of Food Hazards

Molecular identification

Initiates file downloadCharacterization of the dominant bacterial communities during storage of Norway lobster and Norway lobster tails (Nephrops norvegicus) based on 16S rDNA analysis by PCR-DGGE

Initiates file downloadMolecular characterization of Salmonella enterica serovar Enteritidis on retail raw poultry in six provinces and two National cities in China

Initiates file downloadQuantification and viability assays of Toxoplasma gondii in commercial “Serrano” ham samples using magnetic capture real-time qPCR and bioassay techniques

A new set of 16S rRNA universal primers for identification of animal species

Initiates file downloadComparison of the specificity, stability, and PCR efficiency of six rice endogenous sequences for detection analyses of genetically modified rice

Initiates file downloadDevelopment of a real-time PCR procedure for quantification of viable Escherichia coli in populations of E. coli exposed to lactic acid, and the acid tolerance of verotoxigenic E. coli (VTEC) from cattle hides

Initiates file downloadDifferentiation of fish species in Taiwan Strait by PCR-RFLP and lab-on-a-chip system

Initiates file downloadOccurrence of the three major Vibrio species pathogenic for human in seafood products consumed in France using real-time PCR

Initiates file downloadRapid and reliable species identification of scallops by MALDI-TOF mass spectrometry

Initiates file downloadRapid and simultaneous detection of Salmonella, Shigella, and Staphylococcus aureus in fresh pork using a multiplex real-time PCR assay based on immunomagnetic separation

Initiates file downloadReal time loop-mediated isothermal amplification using a portable fluorescence scanner for rapid and simple detection of Vibrio parahaemolyticus

Initiates file downloadSequencing, physical organization and kinetic expression of the patulin biosynthetic gene cluster from Penicillium expansum

Initiates file downloadValidation of real-time PCR for detection of six major pathogens in seafood products

Initiates file downloadA novel hapten and monoclonal-based enzyme-linked immunosorbent assay for sulfonamides in edible animal tissues

Initiates file downloadDetection of five Shiga toxin-producing Escherichia coli genes with multiplex PCR

Initiates file downloadDetection of Toxoplasma gondii and Cyclospora cayetanensis in Mytilus galloprovincialis from Izmir Province coast (Turkey) by Real Time PCR/High-Resolution Melting analysis (HRM)

Initiates file downloadDevelopment of a real-time PCR melt curve assay for simultaneous detection of virulent and antibiotic resistant Salmonella

Initiates file downloadGene cloning, functional expression and characterisation of a novel glycogen branching enzyme from Rhizomucor miehei and its application in wheat breadmaking

Initiates file downloadMolecular methods used to estimate thermal inactivation of a prototype human norovirus: More heat resistant than previously believed?

Initiates file downloadRapid detection and differentiation of human noroviruses using RT-PCR coupled to electrospray ionization mass spectrometry

Initiates file downloadRapid detection of aflatoxin producing fungi in food by real-time quantitative loop-mediated isothermal amplification

Initiates file downloadRapid detection of Shigella and enteroinvasive Escherichia coli in produce enrichments by a conventional multiplex PCR assay

Initiates file downloadQuantitative high-resolution melting PCR analysis for monitoring of fermentation microbiota in sourdough

Initiates file downloadReal-time PCR method combined with immunomagnetic separation for detecting healthy and heat-injured Salmonella Typhimurium on raw duck wings

Initiates file downloadrRNA-based monitoring of the microbiota involved in Fontina PDO cheese production in relation to different stages of cow lactation

Initiates file downloadSurvey of Canadian retail pork chops and pork livers for detection of hepatitis E virus, norovirus, and rotavirus using real time RT-PCR

Initiates file downloadThe use of real-time PCR to study Penicillium chrysogenum growth kinetics on solid food at different water activities

Initiates file downloadA barcode for the authentication of the snappers (Lutjanidae) of the western Atlantic: rDNA 5S or mitochondrial COI?

Initiates file downloadA rapid and simple method for distinguishing two mitten crabs (Eriocheir sinensis and Eriocheir japonica) in Korea using PCR-RFLP and PCR

Initiates file downloadA simple quantum dot-based fluoroimmunoassay method for selective capturing and rapid detection of Salmonella Enteritidis on eggs

Initiates file downloadComparative evaluation of eleven commercial DNA extraction kits for real-time PCR detection of Bacillus anthracis spores in spiked dairy samples

Initiates file downloadDetection of viable Escherichia coli O157:H7 in ground beef by propidium monoazide real-time PCR

Initiates file downloadDevelopment of an immunomagnetic separation method for efficient enrichment of Escherichia coli O157:H7

Initiates file downloadDifferentiation and identification of filamentous fungi by high-throughput FTIR spectroscopic analysis of mycelia


Flow cytometry immunodetection and membrane integrity assessment of Escherichia coli O157:H7 in ready-to-eat pasta salad during refrigerated storage

Direct measurement of 3-chloropropane-1,2-diol fatty acid esters in oils and fats by HPLC method

Initiates file downloadItalian market fish species identification and commercial frauds revealing by DNA sequencing

Initiates file downloadPreparation of magnetic molecularly imprinted polymer for the extraction of melamine from milk followed by liquid chromatography-tandem mass spectrometry

Initiates file downloadRapid and accurate detection of viable Escherichia coli O157:H7 in milk using a combined IMS, sodium deoxycholate, PMA and real-time quantitative PCR process

Initiates file downloadA comparison of shiga-toxin 2 bacteriophage from classical enterohemorrhagic Escherichia coli serotypes and the german E. coli o104:h4 outbreak strain

Initiates file downloadA high-throughput, precipitating colorimetric sandwich ELISA microarray for shiga toxins

Initiates file downloadA new protocol to detect multiple foodborne pathogens with pcr dipstick dna chromatography after a six-hour enrichment culture in a broad-range food pathogen enrichment broth

Initiates file downloadApplication of bacteriophages for detection of foodborne pathogens

Assessments of total and viable Escherichia coli O157:H7 on field and laboratory grown lettuce

Atypical Listeria monocytogenes serotype 4b strains harboring a lineage ii-specific gene cassette

Initiates file downloadCryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi and other intestinal parasites in young children in lobata province, democratic republic of Sa˜o Tome´ and Principe

Initiates file downloadCulture versus PCR for Salmonella species identification in some dairy products and dairy handlers with special concern to its zoonotic importance

Antimicrobial resistance and pcr-ribotyping of Shigella responsible for foodborne outbreaks occurred in southern Brazil

Initiates file downloadDiagnosis of Listeria monocytogenes meningoencephalitis by Real-Time PCR for the hly gene early identification of common-source foodborne virus outbreaks in europe

 Effect of post harvest UV-C treatment on the bacterial diversity of Ataulfo mangoes by PCR DGGE, survival of E. coli and antimicrobial activity

Endemic bacteriophages: a cautionary tale for evaluation of bacteriophage therapy and other interventions for infection control in animals

Initiates file downloadSimultaneous screening of 24 target genes of foodborne pathogens in 35 foodborne outbreaks using multiplex Real-Time SYBR green PCR analysis

Initiates file downloadFunctional screening of antibiotic resistance genes from a representative metagenomic library of food fermenting microbiota

Gene detection and toxin production evaluation of hemolysin BL of Bacillus cereus isolated from milk and dairy products marketed in Brazil

Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP

Genomic characterization of Listeria monocytogenes strains involved in a multistate listeriosis outbreak associated with cantaloupe in US

Initiates file downloadIdentification of Campylobacter fetus by PCR-DNA probe method

Initiates file downloadIdentification of Vibrio isolates by a multiplex PCR assay and rpoB sequence determination

Initiates file downloadImproved method for the detection and quantification of Naegleria fowleri in water and sediment using immunomagnetic separation and Real-Time PCR

Initiates file downloadInsertion element IS3-Based PCR method for subtyping Escherichia coli O157:H7

Initiates file downloadMeta-analysis of pulsed-field gel electrophoresis fingerprints based on a constructed salmonella database

Microbial assessment and prevalence of foodborne pathogens in natural cheeses in Japan

Initiates file downloadMolecular analysis of antimicrobial-susceptible and -resistant escherichia coli from retail meats and human stool and clinical specimens in a rural community setting

Molecular characterization of a novel mosaic tet(S/M) gene encoding tetracycline resistance in foodborne strains of Streptococcus bovis

Molecular identification of Enterocytozoon bieneusi isolates from nigerian children

Multicolor combinatorial probe coding for Real-Time PCR

Multilocus sequence typing of an emerging Cryptosporidium hominis subtype in the United States

Multiplex PCR for distinguishing the most common phase-1 Flagellar antigens of Salmonella spp.

Rapid determination of Escherichia coli O157:H7 lineage types and molecular subtypes by using comparative genomic fingerprinting

Rapid genoserotyping tool for classification of Salmonella serovars

Initiates file downloadRapid identification and quantification of Campylobacter coli and Campylobacter jejuni by cultures and in complex Real- time PCR in pure samples

Rapid screening of Salmonella enterica serovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles

Foodborne Illness Acquired in the United States—Major Pathogens

Serial foodborne Norovirus outbreaks associated with multiple genotypes

Initiates file downloadSimultaneous detection of pathogenic B. cereus, S. aureus and L. monocytogenes by multiplex PCR

Initiates file downloadSurvey of culture, goldengate assay, universal biosensor assay, and 16S rRNA gene sequencing as alternative methods of bacterial pathogen detection

Initiates file downloadThe genome and proteome of a Campylobacter coli bacteriophage vB_CcoM-IBB_35 reveal unusual features

Underdiagnosis of foodborne hepatitis A, the Netherlands, 2008–2010

Use of Norovirus genotype profiles to differentiate origins of foodborne outbreaks

Variation in virulence among clades of Escherichia coli O157:H7 associated with disease outbreaks

Selection tool for foodborne norovirus outbreaks, United States, 1991–2000

A foodborne outbreak of gastroenteritis associated with norwalk-like viruses: first molecular traceback to deli sandwiches contaminated during preparation

Initiates file downloadA new protocol to detect multiple foodborne pathogens with PCR dipstick DNA chromatography after a six-hour enrichment culture in a broad-range food pathogen enrichment broth

Antimicrobial resistance and pcr-ribotyping of Shigella responsible for foodborne outbreaks occurred in southern brazil

Detection of multiple enteric virus strains within a foodborne outbreak of gastroenteritis: an indication of the source of contamination

Initiates file downloadFoodborne norovirus outbreak: the role of an asymptomatic food handler

Microbial assessment and prevalence of foodborne pathogens in natural cheeses in japan

Pyridinyl polythiazole class peptide antibiotic micrococcin p1, secreted by foodborne staphylococcus equorum ws2733, is biosynthesized nonribosomally

Simultaneous, specific and real-time detection of biothreat and frequently encountered food-borne pathogens

A microarray based approach for the identification of common foodborne viruses

An investigation of the diversity of strains of enteroaggregative escherichia coli isolated from cases associated with a large multi-pathogen foodborne outbreak in the UK

Comprehensive and rapid real-time pcr analysis of21 foodborne outbreaks

Epidemiological analysis of strains of Salmonella enterica serotype enteritidis from foodborne outbreaks occurring in italy, 1980-1994

Identification of 8 foodborne pathogens by multicolor combinational probe coding technology in a single real-time PCR

Molecular characterization of a novel mosaic tet(s/m) gene encoding tetracycline resistance in foodborne strains of streptococcus bovis

Simultaneous screening of 24 target genes of foodborne pathogens in 35 foodborne outbreaks using multiplex real-time sybr green pcr analysis

Diagnostic Real-Time PCR assays for the detection of emetic Bacillus cereus strains in foods and recent food-borne outbreaks

Improved template preparation for PCR-based assays for detection of food-borne bacterial pathogens

Molecular characterization of foodborne-associated Staphylococcus aureus strains isolated in Shijiazhuang, China, from 2010 to 2012

PCR-based molecular characterization and insilico analysis of food-borne trematode parasites Paragonimus westermani, Fasciolopsis buski and Fasciola gigantica from northeast India using ITS2 rDNA

Rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and Real-Time PCR

The near-quantitative sampling of genomic DNA from various food-borne Eubacteria

Toward an international standard for PCR-based detection of food-borne thermotolerant campylobacters: validation in a multicenter collaborative trial

Molecular methods to investigate adhesion, transmigration, invasion and intracellular survival of the foodborne pathogen Campylobacter jejuni

Rapid detection of several foodborne pathogens by F0F1-ATPase molecular motor biosensor

Initiates file downloadAn FDA bioinformatics tool for microbial genomics research on molecular characterization of bacterial foodborne pathogens using microarrays

Molecular characterization of class 1 integrons and antimicrobial resistance in Aeromonas strains from foodborne outbreak-suspect samples and environmental sources in Taiwan

Molecular epidemiology of foodborne Hepatitis A outbreaks in the United States, 2003

A molecular marker for evaluating the pathogenic potential of foodborne Listeria monocytogenes

Foodborne disease surveillance needs in Australia: harmonisation of molecular laboratory testing and sharing data from human, animal, and food sources

Molecular epidemiology of Escherichia coli 0157:H7 strains by bacteriophage a restriction fragment length polymorphism analysis: application to a multistate foodborne outbreak and a day-care center cluster

Initiates file downloadAction mechanism of small and large molecule surfactantbased clove oil nanoemulsions against foodborne pathogens and realtime detection of their subpopulations

Occurrence and diversity of integrons and β-lactamase genes among ampicillin-resistant isolates from estuarine waters

Characterization of integrons and tetracycline resistance determinants in Aeromonas spp. isolated from South African aquaculture systems

Performance and mechanism of standard nano-TiO2 (P-25) in photocatalytic disinfection of foodborne microorganisms e Salmonella typhimurium and Listeria monocytogenes

Efficacy of pulsed electric fields for the inactivation of indicator microorganisms and foodborne pathogens in liquids and raw chicken

Restriction fragment length polymorphisms analysis by pulsed-field gel electrophoresis for discrimination of Staphylococcus aureus isolates from foodborne outbreaks

A rapid and sensitive method for detecting foodborne pathogens by capillary electrophoresis-based single-strand conformation polymorphism

Determination of foodborne pathogenic bacteria by multiplex PCR-microchip capillary electrophoresis with genetic algorithm-support vector regression optimization